• When spray drying a biopharmaceutical(BP), its integrity should be maintained upon processing.
• The integrity of a biopharmaceutical can be determined by monitoring its conformational stability through its melting Temperature (T_m) shift.
• Differential Scanning Calorimetry (DSC) and Dynamic Light Scattering (DLS) are two analytical techniques usually employed to determine T_m

However,

1. these require large sample volumes at high concentrations
2. are time-consuming

Differential Scanning Fluorimetry might be an alternative

• allows simultaneous screening of more than one sample;
• at low concentration requirements.

Convenient when dealing with expensive molecules such as biopharmaceuticals!