Knowledge Center

Poster / Apr 25, 2017

On the use of Differential Scanning Fluorimetry to Assess the Impact of Spray Drying on Superoxide Dismutase for Dry Powder Inhaler Formulations

  • Diana Fernandes
RDD Europe 2017
  • When spray drying a biopharmaceutical(BP), its integrity should be maintained upon processing.
  • The integrity of a biopharmaceutical can be determined by monitoring its conformational stability through its melting Temperature (Tm) shift.
  • Differential Scanning Calorimetry (DSC) and Dynamic Light Scattering (DLS) are two analytical techniques usually employed to determine Tm


  1. these require large sample volumes at high concentrations
  2. are time-consuming

Differential Scanning Fluorimetry might be an alternative

  • allows simultaneous screening of more than one sample;
  • at low concentration requirements.

Convenient when dealing with expensive molecules such as biopharmaceuticals!